Download The Wilms’ Tumor (WT1) Gene: Methods and Protocols by Nicholas Hastie PDF

By Nicholas Hastie

This quantity offers a radical evaluation of the Wilms’ Tumour Gene (WT1). The booklet starts with 3 evaluate chapters that disguise the involvement of WT1 in pediatric melanoma, kidney disorder, and tissue improvement and homeostasis. the following few chapters talk about mobile marking and lineage tracing, epicardial mobile method, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools.  the subsequent crew of chapters explores the newest instruments in genomics, molecular biology, and biochemistry. They speak about dissecting transcription issue functionality in mobile loose platforms, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics ways for examining subsequent new release series information. the ultimate bankruptcy discusses protocols for medical trials for immune treatment utilizing anti-WT1 peptides. Written within the hugely winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and pointers on troubleshooting and warding off identified pitfalls.

Practical and thorough, The Wilms’ Tumour (WT1) Gene: tools and Protocols is a invaluable source for somebody who's attracted to the varied methodologies utilized in WT1 research.


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Extra resources for The Wilms’ Tumor (WT1) Gene: Methods and Protocols

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Wagner KD, Wagner N, Wellmann S et al (2003) Oxygen-regulated expression of the Wilms’ tumor suppressor Wt1 involves hypoxia-inducible factor-1 (HIF-1). FASEB J 17:1364–1366 102. Scholz H, Wagner KD, Wagner N (2009) Role of the Wilms’ tumour transcription fac- 103. 104. 105. 106. 107. 108. 109. 110. 111. 112. 113. 114. 115. tor, Wt1, in blood vessel formation. Pflugers Arch 458:315–323 Lee SB, Haber DA (2001) Wilms’ tumor and the WT1 gene. Exp Cell Res 264:74–99 Loeb DM, Sukumar S (2002) The role of WT1 in oncogenesis: tumor suppressor or oncogene?

Nat Med 14(9):931–938 29. Hahn H, Cho YM, Park YS et al (2006) Two cases of isolated diffuse mesangial sclerosis with WT1 mutations. J Korean Med Sci 21(1):160–164 30. Jeanpierre C, Denamur E, Henry I et al (1998) Identification of constitutional WT1 mutations, in patients with isolated diffuse mesangial sclerosis, and analysis of genotype/ phenotype correlations by use of a computerized mutation database. Am J Hum Genet 62(4):824–833 Clinical Aspects of WT1 and the Kidney 31. Brown EJ, Pollak MR, Barua M (2014) Genetic testing for nephrotic syndrome and FSGS in the era of next-generation sequencing.

Histol Histopathol 27:1195–1202 Kirschner KM, Sciesielski LK, Scholz H et al (2010) Wilms’ tumour protein Wt1 stimulates transcription of the gene encoding vascular endothelial cadherin. Pflugers Arch 460:1051–1061 Zhou B, Pu WT (2012) Genetic Cre-loxP assessment of epicardial cell fate using Wt1driven Cre alleles. Circ Res 111:e276–e280 King-Underwood L, Little S, Baker M et al (2005) Wt1 is not essential for hematopoiesis in the mouse. Leuk Res 29:803–812 Herzer U, Crocoll A, Barton D et al (1999) The Wilms’ tumor suppressor gene wt1 is required for development of the spleen.

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